hybridization in situ meaning in Chinese
原位杂交
Examples
- Studies on detection of nosema bombycis by hybridization in situ
家蚕微孢子虫原位杂交诊断技术研究 - Selected one of the 14 strains - s93 , s93 dna was digested partially with sau3a i and 2 ~ 3kb fragments were collected and inserted into puc 18 , then transformed into dh5 a . filtering the clone with hybridization in situ , a 1 kb frament clone has been cloned
使用sau3ai对基因组dna进行不完全酶切，回收2 3kb片段，与puc18质粒连接转化大肠杆菌，利用地高辛标记探针，使用菌落原位杂交筛选转化子；筛选到包含有约1kb外源片段的转化子。 - In this experiment , radio - immunoassay and hybridization in situ were applied to observe the insulinotropic activities of glp - 1 ( 7 - 36 ) nh2 and reveal the mechanisms underlying this process . methods : rat pancreases were removed from 3 - 5 day - old sprague - dawley rats and dissected into 0 . 5mm3 segments and islets were isolated by the collagenase digestion method of wangling et al . thoroughly washed islets and suspended in modified rpmi - 1640 medium supplemented with 10 % fetal bovine serum , and added to 50ml cell culture flasks
方法：胰岛的分离参照王玲等的方法，每次实验取新生3 - 5天sd大鼠，无菌条件下剖腹取出胰腺，剪切为0 . 5mm ~ 3的组织块， v型胶原酶消化30min后，离心洗涤，悬浮于完全培养基，接种入50ml培养瓶，于5 co _ 2 、 95空气条件下培养20h ，转板纯化，接种于96孔培养板培养24h ，按实验要求进行实验。
Related Words
- artificial hybridization
- oligonucleotide hybridization
- somatic hybridization
- colony hybridization
- filter hybridization
- differential hybridization
- introgressive hybridization
- graft hybridization
- provenance hybridization
- background hybridization
- hybridization effect
- hybridization histochemistry
- hybridization of atomic orbitals
- hybridization of nucleic acid